Mathew Casimiro
Mathew Casimiro
Assistant Professor
School of Arts and Sciences
EDUCATION AND TRAINING
1993-1998 Ph.D. Microbiology - University of Sunderland, United Kingdom
Thesis: A study of autotrophic mutants of P. thermocarboxydovorans C2 by Tn5 mutagenesis.
1992-1993 M.Sc. University of Sussex at Brighton, United Kingdom
M.Sc. in Biochemistry (Genetic Manipulation and Molecular Biology).
1989-1992 B.Sc. University of Portsmouth, United Kingdom
B.Sc. (Honors) Molecular Biology, Honors project: Analysis of two temperature sensitive mutants of a Type I restriction endonuclease from Eschericha coli K.
PROFESSIONAL EXPERIENCE
Research and academic appointments
2018-2019 ¾ Professor, Rowan University, Department of Biological Sciences, New Jersey.
Biochemistry-CHEM 07348 6 Fall Semester
RCBC-Introduction to Biochemistry 14440 1C Fall Semester
Biochemistry-CHEM 07348 1 Spring Semester
Introduction to genetics - BIOL 01106 8 Spring Semester
Introduction to genetics - BIOL 01106 9 Spring Semester
2017 Assistant Professor, Pennsylvania Cancer and Regenerative Medicine Research Center, Baruch S. Blumberg Institute, Doylestown, Pennsylvania.
2008-2016 Assistant Professor, Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania.
2006-2008 Instructor, Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania.
2004-2006 Research Fellow, Department of Oncology, Lombardi Cancer Center, Georgetown University, Washington DC.
1999-2004 Visiting Fellow, Unit of Genomic Imprinting, National Institutes of Health, Bethesda, Maryland.
Postdoctoral research fellowship at Laboratory of Mammalian Genes and Development, National Institute of Child Health and Development.
1993-1998 Visiting Lecturer, University of Sunderland.
Demonstrating and tutoring undergraduate students in microbiology, biochemistry, molecular biology and virology.
PEER REVIEWED RESEARCH ARTICLES
1. Casimiro, M.C., Di Sante, G., Di Rocco, A., Loro, E., Pupo, C., Pestell, T.G., Bisetto, S., Velasco-Velázquez, M.A., Jiao, X., Li., Z, Kusminski, C.M., Seifert, E.L., Wang, C., Ly, D., Zheng, B., Shen, C.H., Scherer, P.E, Pestell, R.G. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Research. 2017 Jul 1;77(13):3391-3405.
2. Di Sante, G., Casimiro, M.C., Pestell, T.G., Pestell, R.G. Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy. J Vis Exp. 2016 May
3. Casimiro, M.C., Di Sante, G., Ju, X., Li, Z., Chen, K., Crosariol, M., Yaman, I., Gormley, M., Meng, H., Lisanti, M.P., Pestell, R.G. Cyclin D1 Promotes Androgen-Dependent DNA Damage Repair in Prostate Cancer Cells. Cancer Research. 2016 Jan 15;76(2):329-38.
4. Casimiro, M.C., Di Sante, G., Crosariol, M., Loro, E., Dampier, W., Ertel, A., Yu, Z., Saria, E.A., Papanikolaou, A., Li, Z., Wang, C., Addya, S., Lisanti, M.P., Fortina, P., Cardiff, R.D., Tozeren, A., Knudsen, E.S., Arnold, A., Pestell, R.G. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20;6(11):8525-38.
5. Casimiro M.C., Di Sante, G., Crosariol, M., Loro, E., Dampier, W., Ertel, A., Yu, Z., Saria, E.A., Papanikolaou, A., Li, Z., Wang, C., Addya, S., Lisanti, M.P., Fortina, P., Cardiff, R.D., Tozeren, A., Knudsen, E.S., Arnold, A., Pestell, R.G. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20; 6(11): 8525-38.
6. Di Sante, G., Pestell, T.G., Casimiro, M.C., Bisetto, S., Powell, M.J., Lisanti, M.P., Cordon-Cardo, C., Castillo-Martin, M., Bonal, D.M., Debattisti, V., Chen, K., Wang, L., He, X., McBurney, M.W., Pestell, R.G. Loss of Sirt1 promotes prostatic intraepithelial neoplasia, reduces mitophagy, and delays PARK2 translocation to mitochondria. American Journal of Patholology. 2015 Jan; 185(1): 266-79.
7. Ju, X. and Casimiro, M.C., Gormley, M., Meng, H., Jiao, X., Katiyar, S., Crosariol, M., Chen, K., Wang, M., Quong, A.A., Lisanti MP, Ertel A, Pestell RG. Identification of a cyclin D1 network in prostate cancer that antagonizes epithelial-mesenchymal restraint. Cancer Research. 2014 Jan 15; 74(2): 508-19.
8. Casimiro, M.C., Wang, C., Li, Z., Di Sante, G., Willmart, N.E., Addya, S., Chen, L., Liu, Y., Lisanti, M.P., Pestell, R.G. Cyclin d1 determines estrogen signaling in the mammary gland in vivo. Molecular Endocrinology. 2013 Sep; 27(9): 1415-28.
19. Ju, X., Ertel, A., Casimiro, M.C., Yu, Z., Meng, H., McCue, P.A., Walters, R., Fortina, P., Lisanti, M.P., Pestell, R.G. Novel oncogene-induced metastatic prostate cancer cell lines define human prostate cancer progression signatures. Cancer Research. 2013 Jan 15; 73(2): 978-89.
10. Casimiro, M.C., Pestell R.G. Cyclin d1 induces chromosomal instability. Oncotarget. 2012 Mar; 3(3): 224-5.
11. Casimiro, M.C., Crosariol, M., Loro, E., Ertel, A., Yu, Z., Dampier, W., Saria, E., Papanikolaou, A., Stanek, T., Li, Z, Wang, C., Fortina, P., Addya, S., Tozeren, A., Knudsen, E.S., Arnold, A. and Pestell, R.G. ChIP-Sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice. Journal of Clinical Investigation. 2013 May 1;123(5):2332.
1993-1998 Ph.D. Microbiology - University of Sunderland, United Kingdom
Thesis: A study of autotrophic mutants of P. thermocarboxydovorans C2 by Tn5 mutagenesis.
1992-1993 M.Sc. University of Sussex at Brighton, United Kingdom
M.Sc. in Biochemistry (Genetic Manipulation and Molecular Biology).
1989-1992 B.Sc. University of Portsmouth, United Kingdom
B.Sc. (Honors) Molecular Biology, Honors project: Analysis of two temperature sensitive mutants of a Type I restriction endonuclease from Eschericha coli K.
PROFESSIONAL EXPERIENCE
Research and academic appointments
2018-2019 ¾ Professor, Rowan University, Department of Biological Sciences, New Jersey.
Biochemistry-CHEM 07348 6 Fall Semester
RCBC-Introduction to Biochemistry 14440 1C Fall Semester
Biochemistry-CHEM 07348 1 Spring Semester
Introduction to genetics - BIOL 01106 8 Spring Semester
Introduction to genetics - BIOL 01106 9 Spring Semester
2017 Assistant Professor, Pennsylvania Cancer and Regenerative Medicine Research Center, Baruch S. Blumberg Institute, Doylestown, Pennsylvania.
2008-2016 Assistant Professor, Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania.
2006-2008 Instructor, Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania.
2004-2006 Research Fellow, Department of Oncology, Lombardi Cancer Center, Georgetown University, Washington DC.
1999-2004 Visiting Fellow, Unit of Genomic Imprinting, National Institutes of Health, Bethesda, Maryland.
Postdoctoral research fellowship at Laboratory of Mammalian Genes and Development, National Institute of Child Health and Development.
1993-1998 Visiting Lecturer, University of Sunderland.
Demonstrating and tutoring undergraduate students in microbiology, biochemistry, molecular biology and virology.
PEER REVIEWED RESEARCH ARTICLES
1. Casimiro, M.C., Di Sante, G., Di Rocco, A., Loro, E., Pupo, C., Pestell, T.G., Bisetto, S., Velasco-Velázquez, M.A., Jiao, X., Li., Z, Kusminski, C.M., Seifert, E.L., Wang, C., Ly, D., Zheng, B., Shen, C.H., Scherer, P.E, Pestell, R.G. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Research. 2017 Jul 1;77(13):3391-3405.
2. Di Sante, G., Casimiro, M.C., Pestell, T.G., Pestell, R.G. Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy. J Vis Exp. 2016 May
3. Casimiro, M.C., Di Sante, G., Ju, X., Li, Z., Chen, K., Crosariol, M., Yaman, I., Gormley, M., Meng, H., Lisanti, M.P., Pestell, R.G. Cyclin D1 Promotes Androgen-Dependent DNA Damage Repair in Prostate Cancer Cells. Cancer Research. 2016 Jan 15;76(2):329-38.
4. Casimiro, M.C., Di Sante, G., Crosariol, M., Loro, E., Dampier, W., Ertel, A., Yu, Z., Saria, E.A., Papanikolaou, A., Li, Z., Wang, C., Addya, S., Lisanti, M.P., Fortina, P., Cardiff, R.D., Tozeren, A., Knudsen, E.S., Arnold, A., Pestell, R.G. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20;6(11):8525-38.
5. Casimiro M.C., Di Sante, G., Crosariol, M., Loro, E., Dampier, W., Ertel, A., Yu, Z., Saria, E.A., Papanikolaou, A., Li, Z., Wang, C., Addya, S., Lisanti, M.P., Fortina, P., Cardiff, R.D., Tozeren, A., Knudsen, E.S., Arnold, A., Pestell, R.G. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20; 6(11): 8525-38.
6. Di Sante, G., Pestell, T.G., Casimiro, M.C., Bisetto, S., Powell, M.J., Lisanti, M.P., Cordon-Cardo, C., Castillo-Martin, M., Bonal, D.M., Debattisti, V., Chen, K., Wang, L., He, X., McBurney, M.W., Pestell, R.G. Loss of Sirt1 promotes prostatic intraepithelial neoplasia, reduces mitophagy, and delays PARK2 translocation to mitochondria. American Journal of Patholology. 2015 Jan; 185(1): 266-79.
7. Ju, X. and Casimiro, M.C., Gormley, M., Meng, H., Jiao, X., Katiyar, S., Crosariol, M., Chen, K., Wang, M., Quong, A.A., Lisanti MP, Ertel A, Pestell RG. Identification of a cyclin D1 network in prostate cancer that antagonizes epithelial-mesenchymal restraint. Cancer Research. 2014 Jan 15; 74(2): 508-19.
8. Casimiro, M.C., Wang, C., Li, Z., Di Sante, G., Willmart, N.E., Addya, S., Chen, L., Liu, Y., Lisanti, M.P., Pestell, R.G. Cyclin d1 determines estrogen signaling in the mammary gland in vivo. Molecular Endocrinology. 2013 Sep; 27(9): 1415-28.
19. Ju, X., Ertel, A., Casimiro, M.C., Yu, Z., Meng, H., McCue, P.A., Walters, R., Fortina, P., Lisanti, M.P., Pestell, R.G. Novel oncogene-induced metastatic prostate cancer cell lines define human prostate cancer progression signatures. Cancer Research. 2013 Jan 15; 73(2): 978-89.
10. Casimiro, M.C., Pestell R.G. Cyclin d1 induces chromosomal instability. Oncotarget. 2012 Mar; 3(3): 224-5.
11. Casimiro, M.C., Crosariol, M., Loro, E., Ertel, A., Yu, Z., Dampier, W., Saria, E., Papanikolaou, A., Stanek, T., Li, Z, Wang, C., Fortina, P., Addya, S., Tozeren, A., Knudsen, E.S., Arnold, A. and Pestell, R.G. ChIP-Sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice. Journal of Clinical Investigation. 2013 May 1;123(5):2332.
Rowan University-3/4 Professor (2018-2019).
Biochemistry-CHEM 07348 6 Fall Semester 4-Credit hours
RCBC-Introduction to Biochemistry 14440 1C Fall Semester 3-Credit hours
Biochemistry-CHEM 07348 1 Spring Semester 4-Credit hours
Introduction to genetics - BIOL 01106 8 Spring Semester 4-Credit hours
Introduction to genetics - BIOL 01106 9 Spring Semester 4-Credit hours
Biochemistry-CHEM 07348 6 Fall Semester 4-Credit hours
RCBC-Introduction to Biochemistry 14440 1C Fall Semester 3-Credit hours
Biochemistry-CHEM 07348 1 Spring Semester 4-Credit hours
Introduction to genetics - BIOL 01106 8 Spring Semester 4-Credit hours
Introduction to genetics - BIOL 01106 9 Spring Semester 4-Credit hours
CLINICAL AND BIOTECHNOLOGY ACTIVITIES
2013-2016-Sponsored Research agreement between Thomas Jefferson and Prostagene LLC.
Clinical Trial Study 1. Circulating Tumor Cells derived from blood of PCa patients.
Clinical Trial Study 2. The objective of this study is to perform prostate cancer gene expression characterization of prostate cancer deidentified biospecimens (urine prostate cells, matching biopsy, and prostatectomy specimens).
Clinical activities performed
Study design including IRB approval and liaising with clinical trial manager. Crafting trial documentation e.g. patient consent, sponsored research agreement, budget justification and approvals, scope of work, clinical trial protocol, initial and continued review requirements.
Laboratory activities performed
Prognostic/diagnostic chip-manufacture and analysis. mRNA derived from matched biopsy and prostate tumor samples (deidentified patients) mRNA analysis by Affymetrix array, Nanostring and QT-PCR. Comparative analysis of expression profiles.
2013-2016-Sponsored Research agreement between Thomas Jefferson and Prostagene LLC.
Clinical Trial Study 1. Circulating Tumor Cells derived from blood of PCa patients.
Clinical Trial Study 2. The objective of this study is to perform prostate cancer gene expression characterization of prostate cancer deidentified biospecimens (urine prostate cells, matching biopsy, and prostatectomy specimens).
Clinical activities performed
Study design including IRB approval and liaising with clinical trial manager. Crafting trial documentation e.g. patient consent, sponsored research agreement, budget justification and approvals, scope of work, clinical trial protocol, initial and continued review requirements.
Laboratory activities performed
Prognostic/diagnostic chip-manufacture and analysis. mRNA derived from matched biopsy and prostate tumor samples (deidentified patients) mRNA analysis by Affymetrix array, Nanostring and QT-PCR. Comparative analysis of expression profiles.
Pennsylvania Institute of Stem Cell and Regenerative Medicine-Assistant Professor (2017).
Mentor for postdoctoral research fellows
2015-2017 Dr. Agnese DiRocco: Cyclin D1 regulation of energy homeostasis in the context of aberrant cell cycle regulation in cancer.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul 1;77(13):3391-3405.
2010-2017 Dr. Gabriele DeSanti: Novel function of cyclin D1-an epigenetic reader
• Cyclin D1 regulates gene transcription via an epigenetic reader motif.
Gabriele Di Sante, Mathew C. Casimiro, Di Rocco Agnese, Pupo Claudia, Marco Crosariol, Peter Tompa, Agnes Tantos, Chenguang Wang, Zuoren Yu, Ratna Vadlamudi, Monica Mann and Richard G. Pestell. Manuscript in preparation.
Thomas Jefferson University-Instructor/Assistant Professor Department of Cancer Biology (2006-2016).
Graduate teaching
GC 665 Cell Signaling - Biological function of protein acetylation.
GE 636 Regula Michael Powell (Ph.D. student): Sirt1 function in prostate cancer.
Powell MJ, Casimiro MC, Cordon-Cardo C, He X, Yeow WS, Wang C, McCue PA, McBurney MW, Pestell RG. Disruption of a Sirt1-dependent autophagy checkpoint in
the prostate results in prostatic intraepithelial neoplasia lesion formation. Cancer Res. 2011 Feb 1;71(3):964-75.
Mentor for summer students and clinical fellows
2006-2008 Dr. Andrew Shirley (Surgical Resident at Thomas Jefferson Medical College-2-year rotation): Brg-1 acts as a tumor suppressor through regulation of cyclin D1.
Rao M, Casimiro MC, Lisanti MP, D'Amico M, Wang C, Shirley LA, Leader JE, Liu M, Stallcup M, Engel DA, Murphy DJ, Pestell RG. Inhibition of cyclin D1 gene transcription by Brg-1. Cell Cycle. 2008 Mar 1;7(5):647-55.
2007 Harriet B. Eldredge (Medical student in summer research program): Cyclin D1 regulates energy homeostasis through modulating mitochondrial function.
2012 Jessica Puglies (Undergraduate University of Calabria, Italy).
2013 Ersilia Mirabelli (Undergraduate University of Calabria, Italy).
2015 Claudia Pupo (Undergraduate University of Calabria, Italy).
Mentor for postdoctoral research fellows
2015 Dr. Luca Scarsella: Cyclin D1 and androgen receptor signaling in prostate cancer.
2010-2016 Dr. Gabriele DeSanti
Sirt1 restrains prostate cancer.
• Di Sante G, Pestell TG, Casimiro MC, Bisetto S, Powell MJ, Lisanti MP, Cordon-Cardo C, Castillo-Martin M, Bonal DM, Debattisti V, Chen K, Wang L, He X, McBurney MW, Pestell RG. Loss of Sirt1 promotes prostatic intraepithelial neoplasia, reduces mitophagy, and delays PARK2 translocation to mitochondria. Am J Pathol. 2015 Jan;185(1):266-79.
• Di Sante G, Casimiro MC, Pestell TG, Pestell RG. Time-Lapse Video Microscopy
for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy. J Vis Exp. 2016 May 4;(111).
Sirt1 functions as a key regulator of GnRH neuronal migration.
• Di Sante G, Wang L, Wang C, Jiao X, Casimiro MC, Chen K, Pestell TG, Yaman I, Di Rocco A, Sun X, Horio Y, Powell MJ, He X, McBurney MW, Pestell RG. Sirt1-deficient mice have hypogonadotropic hypogonadism due to defective GnRH neuronal migration. Mol Endocrinol. 2015 Feb;29(2):200-12.
Cell cycle regulation by cyclin D1 and its implications in chromosomal stability.
• Casimiro MC, Di Sante G, Crosariol M, Loro E, Dampier W, Ertel A, Yu Z, Saria EA, Papanikolaou A, Li Z, Wang C, Addya S, Lisanti MP, Fortina P, Cardiff RD, Tozeren A, Knudsen ES, Arnold A, Pestell RG. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20;6(11):8525-38.
• Casimiro MC, Di Sante G, Ju X, Li Z, Chen K, Crosariol M, Yaman I, Gormley M,
Meng H, Lisanti MP, Pestell RG. Cyclin D1 Promotes Androgen-Dependent DNA Damage Repair in Prostate Cancer Cells. Cancer Res. 2016 Jan 15;76(2):329-38.
• Di Sante G, Di Rocco A, Pupo C, Casimiro MC, Pestell RG. Hormone-induced DNA
damage response and repair mediated by cyclin D1 in breast and prostate cancer. Oncotarget. 2017 Jul 20;8(47):81803-81812.
Cyclin D1 regulation of cellular energy homeostasis.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco-Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul1;77(13):3391-3405.
2010-2013 Dr. Emanuele Loro: Mitochondrial morphology and mitophagy in breast cancer.
• Casimiro MC, Crosariol M, Loro E, Ertel A, Yu Z, Dampier W, Saria EA,Papanikolaou A, Stanek TJ, Li Z, Wang C, Fortina P, Addya S, Tozeren A, Knudsen ES, Arnold A, Pestell RG. ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice. J Clin Invest. 2012 Mar;122(3):833-43.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco-Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul1;77(13):3391-3405.
2007-2009 Dr. Francesco Marampon: Oncogenic signaling activates Notch, which promotes mammary progenitor cell expansion and tumorigenesis.
• Marampon F, Casimiro MC, Fu M, Powell MJ, Popov VM, Lindsay J, Zani BM, Ciccarelli C, Watanabe G, Lee RJ, Pestell RG. Nerve Growth factor regulation of cyclin D1 in PC12 cells through a p21RAS extracellular signal-regulated kinase pathway requires cooperative interactions between Sp1 and nuclear factor-kappaB. Mol Biol Cell. 2008 Jun;19(6):2566-78.
2006-2009 Dr. Manran Liu: NF-kB blockade can inhibit oncogenic induced mammary tumor growth.
• Liu M, Ju X, Willmarth NE, Casimiro MC, Ojeifo J, Sakamaki T, Katiyar S, Jiao X, Popov VM, Yu Z, Wu K, Joyce D, Wang C, Pestell RG. Nuclear factor kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo. Am J Pathol. 2009 May;174(5):1910-20.
• Liu M, Sakamaki T, Casimiro MC, Willmarth NE, Quong AA, Ju X, Ojeifo J, Jiao X, Yeow WS, Katiyar S, Shirley LA, Joyce D, Lisanti MP, Albanese C, Pestell RG. The canonical NF-kappaB pathway governs mammary tumorigenesis in transgenic mice and tumor stem cell expansion. Cancer Res. 2010 Dec 15;70(24):10464-73.
2006-2008 Dr. Jaime Lindsay: Role of notch in oncogenic signaling.
• Lindsay J, Jiao X, Sakamaki T, Casimiro MC, Shirley LA, Tran TH, Ju X, Liu M, Li Z, Wang C, Katiyar S, Rao M, Allen KG, Glazer RI, Ge C, Stanley P, Lisanti MP, Rui H, Pestell RG. ErbB2 induces Notch1 activity and function in breast cancer cells. Clin Transl Sci. 2008 Sep;1(2):107-15.
tion of Cell Cycle and Apoptosis - Regulation of Cell cycle.
Mentor for postdoctoral research fellows
2015-2017 Dr. Agnese DiRocco: Cyclin D1 regulation of energy homeostasis in the context of aberrant cell cycle regulation in cancer.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul 1;77(13):3391-3405.
2010-2017 Dr. Gabriele DeSanti: Novel function of cyclin D1-an epigenetic reader
• Cyclin D1 regulates gene transcription via an epigenetic reader motif.
Gabriele Di Sante, Mathew C. Casimiro, Di Rocco Agnese, Pupo Claudia, Marco Crosariol, Peter Tompa, Agnes Tantos, Chenguang Wang, Zuoren Yu, Ratna Vadlamudi, Monica Mann and Richard G. Pestell. Manuscript in preparation.
Thomas Jefferson University-Instructor/Assistant Professor Department of Cancer Biology (2006-2016).
Graduate teaching
GC 665 Cell Signaling - Biological function of protein acetylation.
GE 636 Regula Michael Powell (Ph.D. student): Sirt1 function in prostate cancer.
Powell MJ, Casimiro MC, Cordon-Cardo C, He X, Yeow WS, Wang C, McCue PA, McBurney MW, Pestell RG. Disruption of a Sirt1-dependent autophagy checkpoint in
the prostate results in prostatic intraepithelial neoplasia lesion formation. Cancer Res. 2011 Feb 1;71(3):964-75.
Mentor for summer students and clinical fellows
2006-2008 Dr. Andrew Shirley (Surgical Resident at Thomas Jefferson Medical College-2-year rotation): Brg-1 acts as a tumor suppressor through regulation of cyclin D1.
Rao M, Casimiro MC, Lisanti MP, D'Amico M, Wang C, Shirley LA, Leader JE, Liu M, Stallcup M, Engel DA, Murphy DJ, Pestell RG. Inhibition of cyclin D1 gene transcription by Brg-1. Cell Cycle. 2008 Mar 1;7(5):647-55.
2007 Harriet B. Eldredge (Medical student in summer research program): Cyclin D1 regulates energy homeostasis through modulating mitochondrial function.
2012 Jessica Puglies (Undergraduate University of Calabria, Italy).
2013 Ersilia Mirabelli (Undergraduate University of Calabria, Italy).
2015 Claudia Pupo (Undergraduate University of Calabria, Italy).
Mentor for postdoctoral research fellows
2015 Dr. Luca Scarsella: Cyclin D1 and androgen receptor signaling in prostate cancer.
2010-2016 Dr. Gabriele DeSanti
Sirt1 restrains prostate cancer.
• Di Sante G, Pestell TG, Casimiro MC, Bisetto S, Powell MJ, Lisanti MP, Cordon-Cardo C, Castillo-Martin M, Bonal DM, Debattisti V, Chen K, Wang L, He X, McBurney MW, Pestell RG. Loss of Sirt1 promotes prostatic intraepithelial neoplasia, reduces mitophagy, and delays PARK2 translocation to mitochondria. Am J Pathol. 2015 Jan;185(1):266-79.
• Di Sante G, Casimiro MC, Pestell TG, Pestell RG. Time-Lapse Video Microscopy
for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy. J Vis Exp. 2016 May 4;(111).
Sirt1 functions as a key regulator of GnRH neuronal migration.
• Di Sante G, Wang L, Wang C, Jiao X, Casimiro MC, Chen K, Pestell TG, Yaman I, Di Rocco A, Sun X, Horio Y, Powell MJ, He X, McBurney MW, Pestell RG. Sirt1-deficient mice have hypogonadotropic hypogonadism due to defective GnRH neuronal migration. Mol Endocrinol. 2015 Feb;29(2):200-12.
Cell cycle regulation by cyclin D1 and its implications in chromosomal stability.
• Casimiro MC, Di Sante G, Crosariol M, Loro E, Dampier W, Ertel A, Yu Z, Saria EA, Papanikolaou A, Li Z, Wang C, Addya S, Lisanti MP, Fortina P, Cardiff RD, Tozeren A, Knudsen ES, Arnold A, Pestell RG. Kinase-independent role of cyclin D1 in chromosomal instability and mammary tumorigenesis. Oncotarget. 2015 Apr 20;6(11):8525-38.
• Casimiro MC, Di Sante G, Ju X, Li Z, Chen K, Crosariol M, Yaman I, Gormley M,
Meng H, Lisanti MP, Pestell RG. Cyclin D1 Promotes Androgen-Dependent DNA Damage Repair in Prostate Cancer Cells. Cancer Res. 2016 Jan 15;76(2):329-38.
• Di Sante G, Di Rocco A, Pupo C, Casimiro MC, Pestell RG. Hormone-induced DNA
damage response and repair mediated by cyclin D1 in breast and prostate cancer. Oncotarget. 2017 Jul 20;8(47):81803-81812.
Cyclin D1 regulation of cellular energy homeostasis.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco-Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul1;77(13):3391-3405.
2010-2013 Dr. Emanuele Loro: Mitochondrial morphology and mitophagy in breast cancer.
• Casimiro MC, Crosariol M, Loro E, Ertel A, Yu Z, Dampier W, Saria EA,Papanikolaou A, Stanek TJ, Li Z, Wang C, Fortina P, Addya S, Tozeren A, Knudsen ES, Arnold A, Pestell RG. ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice. J Clin Invest. 2012 Mar;122(3):833-43.
• Casimiro MC, Di Sante G, Di Rocco A, Loro E, Pupo C, Pestell TG, Bisetto S, Velasco-Velázquez MA, Jiao X, Li Z, Kusminski CM, Seifert EL, Wang C, Ly D, Zheng B, Shen CH, Scherer PE, Pestell RG. Cyclin D1 Restrains Oncogene-Induced Autophagy by Regulating the AMPK-LKB1 Signaling Axis. Cancer Res. 2017 Jul1;77(13):3391-3405.
2007-2009 Dr. Francesco Marampon: Oncogenic signaling activates Notch, which promotes mammary progenitor cell expansion and tumorigenesis.
• Marampon F, Casimiro MC, Fu M, Powell MJ, Popov VM, Lindsay J, Zani BM, Ciccarelli C, Watanabe G, Lee RJ, Pestell RG. Nerve Growth factor regulation of cyclin D1 in PC12 cells through a p21RAS extracellular signal-regulated kinase pathway requires cooperative interactions between Sp1 and nuclear factor-kappaB. Mol Biol Cell. 2008 Jun;19(6):2566-78.
2006-2009 Dr. Manran Liu: NF-kB blockade can inhibit oncogenic induced mammary tumor growth.
• Liu M, Ju X, Willmarth NE, Casimiro MC, Ojeifo J, Sakamaki T, Katiyar S, Jiao X, Popov VM, Yu Z, Wu K, Joyce D, Wang C, Pestell RG. Nuclear factor kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo. Am J Pathol. 2009 May;174(5):1910-20.
• Liu M, Sakamaki T, Casimiro MC, Willmarth NE, Quong AA, Ju X, Ojeifo J, Jiao X, Yeow WS, Katiyar S, Shirley LA, Joyce D, Lisanti MP, Albanese C, Pestell RG. The canonical NF-kappaB pathway governs mammary tumorigenesis in transgenic mice and tumor stem cell expansion. Cancer Res. 2010 Dec 15;70(24):10464-73.
2006-2008 Dr. Jaime Lindsay: Role of notch in oncogenic signaling.
• Lindsay J, Jiao X, Sakamaki T, Casimiro MC, Shirley LA, Tran TH, Ju X, Liu M, Li Z, Wang C, Katiyar S, Rao M, Allen KG, Glazer RI, Ge C, Stanley P, Lisanti MP, Rui H, Pestell RG. ErbB2 induces Notch1 activity and function in breast cancer cells. Clin Transl Sci. 2008 Sep;1(2):107-15.
tion of Cell Cycle and Apoptosis - Regulation of Cell cycle.
